biotinylated detector antibody  (Biosynth Carbosynth)

Journal: ChemElectroChem

Article Title: Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer

doi: 10.1002/celc.202300641

Figure Lengend Snippet: Scheme 1. Schematic display of the sandwich-type amperometric immunoplatform developed for the determination of human POSTN assisted by MBs.

Article Snippet: Recombinant human periostin standard (POSTN), mouse antihuman POSTN capture antibody (CAb), and biotinylated mouse anti-human POSTN detector antibody (b-DAb) were purchased as the components of the human Periostin/OSF-2 DuoSet ELISA (Cat. No.: DY3548B, R&D Systems).

Techniques:

Journal: ChemElectroChem

Article Title: Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer

doi: 10.1002/celc.202300641

Figure Lengend Snippet: Figure 1. Real amperometric traces (a) and calibration plot (b) provided by the MBs-assisted immunoplatform for the determination of POSTN standards. In (b) error bars were estimated as the standard deviation (SD) of three replicates.

Article Snippet: Recombinant human periostin standard (POSTN), mouse antihuman POSTN capture antibody (CAb), and biotinylated mouse anti-human POSTN detector antibody (b-DAb) were purchased as the components of the human Periostin/OSF-2 DuoSet ELISA (Cat. No.: DY3548B, R&D Systems).

Techniques: Standard Deviation

Journal: ChemElectroChem

Article Title: Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer

doi: 10.1002/celc.202300641

Figure Lengend Snippet: Figure 2. Amperometric responses measured with the developed immuno- platform for 0 (white bars, B) and 2.5 ngmL 1 POSTN (green bars, S) standard solutions prepared in the absence (“NI” bars) and in the presence of 1 and 0.1 mgmL 1 (1/10) hIgG; 5 and 0.5 mgmL 1 (1/10) Hb; 50 and 5 mgmL 1 (1/ 10) HSA; 10 and 1 ngmL 1 (1/10) TNF; 500 ngmL 1 CDH-17; 50 ngmL 1

Article Snippet: Recombinant human periostin standard (POSTN), mouse antihuman POSTN capture antibody (CAb), and biotinylated mouse anti-human POSTN detector antibody (b-DAb) were purchased as the components of the human Periostin/OSF-2 DuoSet ELISA (Cat. No.: DY3548B, R&D Systems).

Techniques:

Journal: ChemElectroChem

Article Title: Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer

doi: 10.1002/celc.202300641

Figure Lengend Snippet: Figure 3. Results obtained with the developed immunoplatform for the determination of POSTN in cell extracts (a) and secretomes (b) from CRC cultured cells with different metastatic capacities. Western Blotting analysis in cell extracts (c), GAPDH was used as loading control, and protein signal intensities, quantified using the ImageJ software, were normalized according to expression in KM12C. In (a) and (b) error bars were estimated as the SD of three replicates.

Article Snippet: Recombinant human periostin standard (POSTN), mouse antihuman POSTN capture antibody (CAb), and biotinylated mouse anti-human POSTN detector antibody (b-DAb) were purchased as the components of the human Periostin/OSF-2 DuoSet ELISA (Cat. No.: DY3548B, R&D Systems).

Techniques: Cell Culture, Western Blot, Control, Software, Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Subsequent malaria enhances virus-specific T cell immunity in SIV-infected Chinese rhesus macaques

doi: 10.1186/s12964-022-00910-7

Figure Lengend Snippet: T-cell repertoire diversity and SIV-specific immune response in malaria- and SIV-infected monkeys. A Diversity index differences with respect to the 8th week of SIV infection (corresponding to malaria introduction in the S + P group). Repertoire diversity was restored in the S + P group but not in the P + S group. A significant difference between the S + P and P + S groups was observed for the Shannon diversity index. P -values computed using ANOVA. B Ratio of SIV-specific clonotype frequency with respect to the control point (8th week of SIV infection, corresponding to malaria introduction in the S + P group). The S + P group, but not the P + S group, was characterized by a persistently higher SIV-specific clonotype frequency. P -values were calculated using ANOVA. C New SIV-specific clonotype production. Fractions of existing (detected at previous sampling point) and new SIV-specific clonotypes shown for each monkey in the P + S, S and S + P groups. A significant increase in new SIV-specific clonotype production was observed in the S + P group during the whole period of Plasmodium infection (at 11–17 weeks and 50 weeks post SIV infection). D Frequency of IFN-g-producing SIV Gag-specific PBMCs. Number of IFN-γ-producing PBMCs against pooled SIV Gag peptides analyzed by ELISPOT. The sample sizes were 6, 4 and 5 on day 49 and 4, 3, and 5 on day 161 of the S, P + S and S + P groups, respectively. SFC: spot-forming cells

Article Snippet: The plates were washed with PBST (PBS containing 0.05% Tween-20), and a biotinylated anti-IFN-γ polyclonal detector antibody (BD Biosciences) was added.

Techniques: Infection, Sampling, Enzyme-linked Immunospot